#2417
Store at –20°C
Syntaxin 6
Antibody
rev. 03/08/11
n 100 µl
(10 western blots)
W, IP
Endogenous
H, M, R, Mk 32 kDa
Rabbit**
Background: Syntaxin 6 is a ubiquitously expressed S25C
family member of the SNARE proteins (1,2). The protein has
an amino-terminal H1 domain followed by an H2 SNARE
domain and a carboxy-terminal membrane anchor (3). Syn-
taxin 6 is localized at the trans-Golgi and within endosomes
and regulates membrane trafficking by partnering with a
variety of other SNARE proteins (3-5). Depending on cell
type and SNARE parter, syntaxin 6 has been found to be
involved in the regulation of GLUT4 trafficking, neutrophil
exocytosis and granule secretion (6-10).
Specificity/Sensitivity: Syntaxin 6 Antibody detects
endogenous levels of total syntaxin 6 protein.
Source/Purification: Polyclonal antibodies are produced
by immunizing animals with a synthetic peptide (KLH-cou-
pled) corresponding residue surrounding Tyr140 of mouse
syntaxin 6 protein. Antibodies are purified by protein A and
peptide affinity chromatography.
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.
*Species cross-reactivity is determined by Western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000
Immunoprecipitation 1:50
For application specific protocols please see the web
page for this product at www.cellsignal.com.
Please visit www.cellsignal.com for a complete listing
of recommended companion products.
Background References:
(1) Bock, J.B. et al. (2001) Nature 409, 839–841.
(2) Bock, J.B. et al. (1996) J. Biol. Chem. 271,
17961–17965.
(3) Wendler, F. and Tooze, S. (2001) Traffic 2, 606–611.
(4) Bock, J.B. et al. (1997) Mol. Biol. Cell 8, 1261–1271.
(5) Mallard, F. et al. (2002) J. Cell Biol. 156, 653–664.
(6) Perera, H.K. et al. (2003) Mol. Biol. Cell 14, 2946–2958.
(7) Shewan, A.M. et al. (2003) Mol. Biol. Cell 14, 973–986.
(8) Martín-Martín, B. et al. (2000) Blood 96, 2574–25783.
(9) Wendler, F. et al. (2001) Mol. Biol. Cell 12, 1699–1709.
(10) Kuliawat, R. et al. (2004) Mol. Biol. Cell 15, 1690–1701.
Orders
n
877-616-CELL (2355)
Support
n
877-678-TECH (8324)
Web
n
www.cellsignal.com
Applications
Species Cross-Reactivity*
Molecular Wt. Source
IMPORTANT: For Western blots, incubate membrane with diluted antibody in 5% w/v w/v nonfat dry
milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
3
kDa
Syntaxin 6
Hek293
A172
NIH/3T3
Neuro2A
PC12
COS
200
140
100
80
60
50
40
30
10
20
Western blot analysis of extracts from various cell lines, using
Syntaxin 6 Antibody.
kDa
IgG
Syntaxin 6
200
140
100
80
60
50
40
30
20
10
Lysate
#2417
#2417 + Lysate
Western blot analysis of A172 lysate (lane1) and immunoprecip-
itates generated in the absence or presence of A172 cell lysate
(lanes 2 and 3), using Syntaxin 6 Antibody both as precipitation
and probing antibody.
Entrez-Gene ID #10228
Swiss-Prot Acc. #O43752
Species Cross-Reactivity Key: H—human M—mouse R—rat Hm—hamster Mk—monkey Mi—mink C—chicken Dm—D. melanogaster X—Xenopus Z—zebrafish B—bovine
Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans Hr—horse All—all species expected
Species enclosed in parentheses are predicted to react based on 100% homology.
Applications Key: W—Western IP—Immunoprecipitation IHC—Immunohistochemistry ChIP—Chromatin Immunoprecipitation IF—Immunofluorescence F—Flow cytometry E-P—ELISA-Peptide
This product is intended for research purposes only. This product is not intended to be used for
therapeutic or diagnostic purposes in humans or animals.
© 2011 Cell Signaling Technology, Inc.
Cell Signaling Technology
®
is a trademark of Cell Signaling Technology, Inc.
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