#2456
Store at –20°C
PSMA3 Antibody
rev. 09/16/10
kDa
PSMA3
NIH/3T3
PC12
COS
Hek293
200
140
100
80
60
50
40
30
20
10
Western blot analysis of extracts from various cell lines using
PMSA3 Antibody.
n 100 µl
(10 western blots)
W
Endogenous
H, M, R, Mk 28 kDa
Rabbit**
Background: The 20S proteasome is the major proteolytic
enzyme complex involved in intracellular protein degrada-
tion. It consists of four stacked rings, each with 7 distinct
subunits. The two outer layers are identical rings composed
of α subunits (called PSMAs), and the two inner layers are
identical rings composed of β subunits. While the catalytic
sites are located on the β rings (1–3), the α subunits are
important for assembly and as binding sites for regulatory
proteins (4). Seven different α and 10 different β protea-
some genes have been identified in mammals (5).
PA700, PA28 and PA200 are three major protein complexes
that function as activators of the 20S proteasome. PA700
binds polyubiquitin with high affinity and associates with
the 20S proteasome to form the 26S proteasome which pref-
erentially degrades polyubiquitinylated proteins (1–3). The
proteasome has a broad substrate spectrum which includes
cell cycle regulators, signaling molecules, tumor suppres-
sors and transcription factors. By controlling the degradation
of these intracellular proteins, the proteasome functions
in cell cycle regulation, cancer development, immune
response, protein folding and disease progression (6–9).
Specificity/Sensitivity: PSMA3 Antibody detects endog-
enous levels of total PSMA3 protein.
Source/Purification: Polyclonal antibodies are produced
by immunizing animals with a synthetic peptide correspond-
ing to residues surrounding Val228 of human PSMA3 protein.
Antibodies are purified by protein A and peptide affinity
chromatography.
Storage: Supplied in 10mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C.
Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000
For application specific protocols please see the web
page for this product at www.cellsignal.com.
Please visit www.cellsignal.com for a complete listing
of recommended companion products.
Orders
n
877-616-CELL (2355)
Support
n
877-678-TECH (8324)
Web
n
www.cellsignal.com
Applications
Species Cross-Reactivity*
Molecular Wt.
Source
© 2010 Cell Signaling Technology, Inc.
Background References:
(1) Dahlmann, B. (2005) Essays Biochem. 41, 31–48.
(2) Pickart, C.M. and Cohen, R.E. (2004) Nat. Rev. Mol.
Cell Biol. 5, 177–187.
(3) Nandi, D. et al. (2006) J. Biosci. 31, 137–155.
(4) Lupas, A. et al. (1993) Enzyme Protein 47, 252–273.
(5) Monaco, J.J. and Nandi, D. (1995) Annu Rev Genet 29,
729–754.
(6) Murray, A.W. (2004) Cell 116, 221–234.
(7) Ciechanover, A. (2006) Proc. Am. Thorac. Soc. 3,
21–31.
(8) Wang, J. and Maldonado, M.A. (2006) Cell. Mol. Im-
munol. 3, 255–261.
(9) Rubinsztein, D.C. (2006) Nature 443, 780–786.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v nonfat dry milk,
1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
3
Swiss-Prot Acc. #P25788
This product is intended for research purposes only. This product is not intended to be used for
therapeutic or diagnostic purposes in humans or animals.
Species Cross-Reactivity Key: H—human M—mouse R—rat Hm—hamster Mk—monkey Mi—mink C—chicken Dm—D. melanogaster X—Xenopus Z—zebrafish B—bovine
Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans Hr—horse All—all species expected
Species enclosed in parentheses are predicted to react based on 100% homology.
Applications Key: W—Western IP—Immunoprecipitation IHC—Immunohistochemistry ChIP—Chromatin Immunoprecipitation IF—Immunofluorescence F—Flow cytometry E-P—ELISA-Peptide
(1 pages)
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